methyl-DNAshape: High-throughput prediction server for methylated DNA shape features
DNA methylation is known to affect the interactions with DNA-binding proteins,
although detailed molecular insights of how cytosine methylation interferes with
binding events are only known yet for very few DNA binding proteins.
DNA methylation can affect direct contacts of proteins with the major groove,
affect interactions with the minor groove through DNA structural changes,
or alter the chromatin structure. Since only a small number of structures
of DNA targets and protein-DNA complexes with cytosine methylation are available
in the Protein Data Bank, we developed methyl-DNAshape that uses the mining of all-atom
Monte Carlo simulations to predict the effect of cytosine methylation on the DNA
shape features minor groove width (MGW), Roll, propeller twist (ProT),
and helix twist (HelT) in comparison to the prediction of those features
in unmethylated DNA using DNAshape.
Click here for methyl-DNAshape prediction.
Click here for methyl-DNAshape prediction.